In the experiment, the technique used, Raster image correlation spectroscopy (RICS), enables observation of a greater area of a biological system through measuring the diffusion of fluorescently labelled molecules from stacks of confocal microscopy images.
Utilising gold nanoparticles prevents photobleaching, so that cells can be viewed for a longer time period.
The ventus 532 provides the correct wavelength for excitation of gold nanoparticles and is a scientific laser with high specifications. It offers power up to 1.5 W, noise <0.15% RMS and power stability of <0.4% RMS within a rugged design, perfect for spectroscopy applications.
To read the full paper, please visit: https://livrepository.liverpool.ac.uk/2014042/1/PhRICS%20published%20version.pdf
